Anti-Human POLQ Polyclonal Antibody (HF853014)

Name: Anti-Human POLQ Polyclonal Antibody
Brand: abinScience
SKU: HF853014
Price: 168 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

HF853014

Description

Anti-Human POLQ Polyclonal Antibody (HF853014) is a rabbit polyclonal antibody detecting POLQ in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human POLQ (Met1-Met894).

Cible

POLH, DNA polymerase eta, DNA polymerase theta, POLQ

Purification

Purified by antigen affinity column.

Numéro d'accès

O75417

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

DNA polymerase theta (POLQ) is a ~289 kDa protein. Low-fidelity DNA polymerase with a helicase activity that promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery required to repair double-strand breaks in DNA during mitosis. MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions, some of them leading to cellular transformation. MMEJ is required during mitosis to repair persistent double-strand breaks that originate in S-phase. Although error-prone, MMEJ protects against chromosomal instability and tumorigenesis. The polymerase acts by binding directly the 2 ends of resected double-strand breaks, allowing microhomologous sequences in the overhangs to form base pairs.

1. Seki, M. et al. (2003) Nucleic acids research 31, 6117-26. PMID: 14576298

2. Arana, ME. et al. (2008) Nucleic acids research 36, 3847-56. PMID: 18503084

3. Yoon, JH. et al. (2014) The Journal of biological chemistry 289, 13177-85. PMID: 24648516

4. Ceccaldi, R. et al. (2015) Nature 518, 258-62. PMID: 25642963

5. Kent, T. et al. (2015) Nature structural & molecular biology 22, 230-7. PMID: 25643323

6. Zahn, KE. et al. (2015) Nature structural & molecular biology 22, 304-11. PMID: 25775267

7. Newman, JA. et al. (2015) Structure (London, England : 1993) 23, 2319-2330. PMID: 26636256

8. Kent, T. et al. (2016) eLife 5. PMID: 27311885

9. Kent, T. et al. (2016) Nucleic acids research 44, 9381-9392. PMID: 27591252

10. Wang, Z. et al. (2019) The Journal of biological chemistry 294, 3909-3919. PMID: 30655289

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Human POLQ Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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