Anti-Human POLQ Polyclonal Antibody (HF853014)

Name: Anti-Human POLQ Polyclonal Antibody
Brand: abinScience
SKU: HF853014
Price: 168 USD
Availability: InStock

Даташит Поделиться продуктом Копировать информацию

Цена(USD):

Спецификация:

50ug
100ug

Количество:

Свяжитесь с нами

Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HF853014

Описание

Anti-Human POLQ Polyclonal Antibody (HF853014) is a rabbit polyclonal antibody detecting POLQ in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Реактивность видов

Human

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human POLQ (Met1-Met894).

Цель

POLH, DNA polymerase eta, DNA polymerase theta, POLQ

Очистка

Purified by antigen affinity column.

Номер доступа

O75417

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

DNA polymerase theta (POLQ) is a ~289 kDa protein. Low-fidelity DNA polymerase with a helicase activity that promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery required to repair double-strand breaks in DNA during mitosis. MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions, some of them leading to cellular transformation. MMEJ is required during mitosis to repair persistent double-strand breaks that originate in S-phase. Although error-prone, MMEJ protects against chromosomal instability and tumorigenesis. The polymerase acts by binding directly the 2 ends of resected double-strand breaks, allowing microhomologous sequences in the overhangs to form base pairs.

1. Seki, M. et al. (2003) Nucleic acids research 31, 6117-26. PMID: 14576298

2. Arana, ME. et al. (2008) Nucleic acids research 36, 3847-56. PMID: 18503084

3. Yoon, JH. et al. (2014) The Journal of biological chemistry 289, 13177-85. PMID: 24648516

4. Ceccaldi, R. et al. (2015) Nature 518, 258-62. PMID: 25642963

5. Kent, T. et al. (2015) Nature structural & molecular biology 22, 230-7. PMID: 25643323

6. Zahn, KE. et al. (2015) Nature structural & molecular biology 22, 304-11. PMID: 25775267

7. Newman, JA. et al. (2015) Structure (London, England : 1993) 23, 2319-2330. PMID: 26636256

8. Kent, T. et al. (2016) eLife 5. PMID: 27311885

9. Kent, T. et al. (2016) Nucleic acids research 44, 9381-9392. PMID: 27591252

10. Wang, Z. et al. (2019) The Journal of biological chemistry 294, 3909-3919. PMID: 30655289

Примечание

For research use only.

Изображения

Ссылки

Даташит

Anti-Human POLQ Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа