Anti-Human POLR1A Recombinant Antibody (SAA1537) (HF629013)

Name: Anti-Human POLR1A Recombinant Antibody (SAA1537)
Brand: abinScience
SKU: HF629013
Price: 196 USD
Availability: InStock

Datasheet Compartir producto Copiar información

Precio(USD):

Especificación:

50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HF629013

Reactividad de especies

Human

Aplicaciones

ELISA

Especie huésped

Mouse

Clonalidad

Monoclonal

Isotipo

IgG2a, kappa

ID de clon

SAA1537

Objetivo

RPA194, POLR1A, RNA polymerase I 194 kDa subunit, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit RPA1, RNA polymerase I subunit A1, A190, DNA-directed RNA polymerase I subunit A

Nivel de endotoxinas

Please contact with the lab for this information.

Pureza

>95% as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

O95602

RRID

Anti-Human POLR1A Antibody (SAA1537) (abinScience Cat# HF629013, RRID:AB_3727298)

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Fondo

DNA-directed RNA polymerase I subunit RPA1 (POLR1A) is a ~194 kDa protein. Catalytic core component of RNA polymerase I (Pol I), a DNA-dependent RNA polymerase which synthesizes ribosomal RNA precursors using the four ribonucleoside triphosphates as substrates. Transcribes 47S pre-rRNAs from multicopy rRNA gene clusters, giving rise to 5.8S, 18S and 28S ribosomal RNAs. Pol I-mediated transcription cycle proceeds through transcription initiation, transcription elongation and transcription termination stages. During transcription initiation, Pol I pre-initiation complex (PIC) is recruited by the selectivity factor 1 (SL1/TIF-IB) complex bound to the core promoter that precedes an rDNA repeat unit. The PIC assembly bends the promoter favoring the formation of the transcription bubble and promoter escape.

1. Miller, G. et al. (2001) The EMBO journal 20, 1373-82. PMID: 11250903

2. Panov, KI. et al. (2001) Molecular and cellular biology 21, 2641-9. PMID: 11283244

3. Panov, KI. et al. (2006) The EMBO journal 25, 3310-22. PMID: 16858408

4. Zhao, D. et al. (2021) Cell discovery 7, 97. PMID: 34671025

5. Misiaszek, AD. et al. (2021) Nature structural & molecular biology 28, 997-1008. PMID: 34887565

6. Daiß, JL. et al. (2022) Life science alliance 5. PMID: 36271492

Nota

For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Anti-Human POLR1A Antibody (SAA1537)

Referencias

Datasheet

Anti-Human POLR1A Recombinant Antibody (SAA1537).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo