Anti-Human POLR1A Recombinant Antibody (SAA1537) (HF629013)

Name: Anti-Human POLR1A Recombinant Antibody (SAA1537)
Brand: abinScience
SKU: HF629013
Price: 196 USD
Availability: InStock

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Prix(USD) :

Spécification :

50ug
100ug
1mg

Nombre :

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HF629013

Reactivité des espèces

Human

Applications

ELISA

Espèce hôte

Mouse

Clonalité

Monoclonal

Isotype

IgG2a, kappa

ID de clone

SAA1537

Cible

RPA194, POLR1A, RNA polymerase I 194 kDa subunit, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit RPA1, RNA polymerase I subunit A1, A190, DNA-directed RNA polymerase I subunit A

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

O95602

RRID

Anti-Human POLR1A Antibody (SAA1537) (abinScience Cat# HF629013, RRID:AB_3727298)

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Contexte

DNA-directed RNA polymerase I subunit RPA1 (POLR1A) is a ~194 kDa protein. Catalytic core component of RNA polymerase I (Pol I), a DNA-dependent RNA polymerase which synthesizes ribosomal RNA precursors using the four ribonucleoside triphosphates as substrates. Transcribes 47S pre-rRNAs from multicopy rRNA gene clusters, giving rise to 5.8S, 18S and 28S ribosomal RNAs. Pol I-mediated transcription cycle proceeds through transcription initiation, transcription elongation and transcription termination stages. During transcription initiation, Pol I pre-initiation complex (PIC) is recruited by the selectivity factor 1 (SL1/TIF-IB) complex bound to the core promoter that precedes an rDNA repeat unit. The PIC assembly bends the promoter favoring the formation of the transcription bubble and promoter escape.

1. Miller, G. et al. (2001) The EMBO journal 20, 1373-82. PMID: 11250903

2. Panov, KI. et al. (2001) Molecular and cellular biology 21, 2641-9. PMID: 11283244

3. Panov, KI. et al. (2006) The EMBO journal 25, 3310-22. PMID: 16858408

4. Zhao, D. et al. (2021) Cell discovery 7, 97. PMID: 34671025

5. Misiaszek, AD. et al. (2021) Nature structural & molecular biology 28, 997-1008. PMID: 34887565

6. Daiß, JL. et al. (2022) Life science alliance 5. PMID: 36271492

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human POLR1A Antibody (SAA1537)

Reférences

Fiche technique

Anti-Human POLR1A Recombinant Antibody (SAA1537).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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