Anti-Human POLR1A Polyclonal Antibody (HF629014)

Name: Anti-Human POLR1A Polyclonal Antibody
Brand: abinScience
SKU: HF629014
Price: 168 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

HF629014

Description

Anti-Human POLR1A Polyclonal Antibody (HF629014) is a rabbit polyclonal antibody detecting POLR1A in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human POLR1A (Asp438-Ala628).

Cible

DNA-directed RNA polymerase I subunit RPA1, RNA polymerase I subunit A1, 2.7.7.6, A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, RNA polymerase I 194 kDa subunit, RPA194, POLR1A

Purification

Purified by antigen affinity column.

Numéro d'accès

O95602

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

DNA-directed RNA polymerase I subunit RPA1 (POLR1A) is a ~194 kDa protein. Catalytic core component of RNA polymerase I (Pol I), a DNA-dependent RNA polymerase which synthesizes ribosomal RNA precursors using the four ribonucleoside triphosphates as substrates. Transcribes 47S pre-rRNAs from multicopy rRNA gene clusters, giving rise to 5.8S, 18S and 28S ribosomal RNAs. Pol I-mediated transcription cycle proceeds through transcription initiation, transcription elongation and transcription termination stages. During transcription initiation, Pol I pre-initiation complex (PIC) is recruited by the selectivity factor 1 (SL1/TIF-IB) complex bound to the core promoter that precedes an rDNA repeat unit. The PIC assembly bends the promoter favoring the formation of the transcription bubble and promoter escape.

1. Miller, G. et al. (2001) The EMBO journal 20, 1373-82. PMID: 11250903

2. Panov, KI. et al. (2001) Molecular and cellular biology 21, 2641-9. PMID: 11283244

3. Panov, KI. et al. (2006) The EMBO journal 25, 3310-22. PMID: 16858408

4. Zhao, D. et al. (2021) Cell discovery 7, 97. PMID: 34671025

5. Misiaszek, AD. et al. (2021) Nature structural & molecular biology 28, 997-1008. PMID: 34887565

6. Daiß, JL. et al. (2022) Life science alliance 5. PMID: 36271492

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Human POLR1A Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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