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| カタログ番号 | HF629014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human POLR1A Polyclonal Antibody (HF629014) is a rabbit polyclonal antibody detecting POLR1A in ELISA, IHC, WB. Suitable for Human.
Highlights
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| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human POLR1A (Asp438-Ala628). | ||||||||
| ターゲット | DNA-directed RNA polymerase I subunit RPA1, RNA polymerase I subunit A1, 2.7.7.6, A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, RNA polymerase I 194 kDa subunit, RPA194, POLR1A | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | O95602 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | DNA-directed RNA polymerase I subunit RPA1 (POLR1A) is a ~194 kDa protein. Catalytic core component of RNA polymerase I (Pol I), a DNA-dependent RNA polymerase which synthesizes ribosomal RNA precursors using the four ribonucleoside triphosphates as substrates. Transcribes 47S pre-rRNAs from multicopy rRNA gene clusters, giving rise to 5.8S, 18S and 28S ribosomal RNAs. Pol I-mediated transcription cycle proceeds through transcription initiation, transcription elongation and transcription termination stages. During transcription initiation, Pol I pre-initiation complex (PIC) is recruited by the selectivity factor 1 (SL1/TIF-IB) complex bound to the core promoter that precedes an rDNA repeat unit. The PIC assembly bends the promoter favoring the formation of the transcription bubble and promoter escape. 1. Miller, G. et al. (2001) The EMBO journal 20, 1373-82. PMID: 11250903 2. Panov, KI. et al. (2001) Molecular and cellular biology 21, 2641-9. PMID: 11283244 3. Panov, KI. et al. (2006) The EMBO journal 25, 3310-22. PMID: 16858408 4. Zhao, D. et al. (2021) Cell discovery 7, 97. PMID: 34671025 5. Misiaszek, AD. et al. (2021) Nature structural & molecular biology 28, 997-1008. PMID: 34887565 6. Daiß, JL. et al. (2022) Life science alliance 5. PMID: 36271492 | ||||||||
| 注意事項 | For research use only. |
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