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Anti-Human POLR3D Polyclonal Antibody (HY534014)

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Vista general
Número de catálogoHY534014
Descripción
Anti-Human POLR3D Polyclonal Antibody (HY534014) is a rabbit polyclonal antibody detecting POLR3D in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human POLR3D (Val249-Arg398).
Objetivo DNA-directed RNA polymerase III subunit RPC4, RNA polymerase III subunit C4, DNA-directed RNA polymerase III subunit D, Protein BN51, RNA polymerase III 47 kDa subunit, RPC53 homolog, POLR3D, BN51, BN51T
Purificación Purified by antigen affinity column.
Número de acceso P05423
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

DNA-directed RNA polymerase III subunit RPC4 (POLR3D) is a ~44 kDa protein. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci. Assembles with POLR3E/RPC5 forming a subcomplex that binds the Pol III core. Enables recruitment of Pol III at transcription initiation site and drives transcription initiation from both type 2 and type 3 DNA promoters. Required for efficient transcription termination and reinitiation.

1. Hu, P. et al. (2002) Molecular and cellular biology 22, 8044-55. PMID: 12391170
2. Canella, D. et al. (2010) Genome research 20, 710-21. PMID: 20413673
3. Girbig, M. et al. (2021) Nature structural & molecular biology 28, 210-219. PMID: 33558764
4. Hou, H. et al. (2021) Nature communications 12, 6135. PMID: 34675218
5. Van Bortle, K. et al. (2022) Nature communications 13, 3007. PMID: 35637192
6. Ablasser, A. et al. (2009) Nature immunology 10, 1065-72. PMID: 19609254
7. Chiu, YH. et al. (2009) Cell 138, 576-91. PMID: 19631370
Nota For research use only.
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Referencias
Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human POLR3D Polyclonal Antibody

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