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Anti-Human POLR3D Polyclonal Antibody (HY534014)

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Aperçu
Numéro de catalogueHY534014
Description
Anti-Human POLR3D Polyclonal Antibody (HY534014) is a rabbit polyclonal antibody detecting POLR3D in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman, Mouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human POLR3D (Val249-Arg398).
Cible DNA-directed RNA polymerase III subunit RPC4, RNA polymerase III subunit C4, DNA-directed RNA polymerase III subunit D, Protein BN51, RNA polymerase III 47 kDa subunit, RPC53 homolog, POLR3D, BN51, BN51T
Purification Purified by antigen affinity column.
Numéro d'accès P05423
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

DNA-directed RNA polymerase III subunit RPC4 (POLR3D) is a ~44 kDa protein. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci. Assembles with POLR3E/RPC5 forming a subcomplex that binds the Pol III core. Enables recruitment of Pol III at transcription initiation site and drives transcription initiation from both type 2 and type 3 DNA promoters. Required for efficient transcription termination and reinitiation.

1. Hu, P. et al. (2002) Molecular and cellular biology 22, 8044-55. PMID: 12391170
2. Canella, D. et al. (2010) Genome research 20, 710-21. PMID: 20413673
3. Girbig, M. et al. (2021) Nature structural & molecular biology 28, 210-219. PMID: 33558764
4. Hou, H. et al. (2021) Nature communications 12, 6135. PMID: 34675218
5. Van Bortle, K. et al. (2022) Nature communications 13, 3007. PMID: 35637192
6. Ablasser, A. et al. (2009) Nature immunology 10, 1065-72. PMID: 19609254
7. Chiu, YH. et al. (2009) Cell 138, 576-91. PMID: 19631370
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-Human POLR3D Polyclonal Antibody

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