Anti-Human POLR3D Polyclonal Antibody (HY534014)

Name: Anti-Human POLR3D Polyclonal Antibody
Brand: abinScience
SKU: HY534014
Price: 168 USD
Availability: InStock

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Каталожный номер

HY534014

Описание

Anti-Human POLR3D Polyclonal Antibody (HY534014) is a rabbit polyclonal antibody detecting POLR3D in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Реактивность видов

Human, Mouse

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human POLR3D (Val249-Arg398).

Цель

DNA-directed RNA polymerase III subunit RPC4, RNA polymerase III subunit C4, DNA-directed RNA polymerase III subunit D, Protein BN51, RNA polymerase III 47 kDa subunit, RPC53 homolog, POLR3D, BN51, BN51T

Очистка

Purified by antigen affinity column.

Номер доступа

P05423

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

DNA-directed RNA polymerase III subunit RPC4 (POLR3D) is a ~44 kDa protein. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci. Assembles with POLR3E/RPC5 forming a subcomplex that binds the Pol III core. Enables recruitment of Pol III at transcription initiation site and drives transcription initiation from both type 2 and type 3 DNA promoters. Required for efficient transcription termination and reinitiation.

1. Hu, P. et al. (2002) Molecular and cellular biology 22, 8044-55. PMID: 12391170

2. Canella, D. et al. (2010) Genome research 20, 710-21. PMID: 20413673

3. Girbig, M. et al. (2021) Nature structural & molecular biology 28, 210-219. PMID: 33558764

4. Hou, H. et al. (2021) Nature communications 12, 6135. PMID: 34675218

5. Van Bortle, K. et al. (2022) Nature communications 13, 3007. PMID: 35637192

6. Ablasser, A. et al. (2009) Nature immunology 10, 1065-72. PMID: 19609254

7. Chiu, YH. et al. (2009) Cell 138, 576-91. PMID: 19631370

Примечание

For research use only.

Изображения

Ссылки

Даташит

Anti-Human POLR3D Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа

Anti-Human POLR3D Polyclonal Antibody (HY534014)

Обзор

Изображения

Ссылки

Даташит

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