Anti-Human POLR3G Polyclonal Antibody (HT290014)

Name: Anti-Human POLR3G Polyclonal Antibody
Brand: abinScience
SKU: HT290014
Price: 96 USD
Availability: InStock

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Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HT290014

Description

Anti-Human POLR3G Polyclonal Antibody (HT290014) is a rabbit polyclonal antibody detecting POLR3G in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human, Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human POLR3G (Met1-Met110).

Cible

DNA-directed RNA polymerase III subunit RPC7, RNA polymerase III subunit C7, DNA-directed RNA polymerase III subunit G, RNA polymerase III 32 kDa apha subunit, RPC32-alpha, RNA polymerase III 32 kDa subunit, RPC32, POLR3G

Purification

Purified by antigen affinity column.

Numéro d'accès

O15318

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

DNA-directed RNA polymerase III subunit RPC7 (POLR3G) is a ~25 kDa protein. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci. Acts as a long tether that bridges POLR3C/RPC3-POLR3F/RPC6-POLR3G/RPC7 heterotrimer and the mobile stalk of Pol III, coordinating the dynamics of Pol III stalk and clamp modules during the transition from apo to elongation state. Pol III exists as two alternative complexes defined by the mutually exclusive incorporation of subunit POLR3G/RPC7alpha or POLR3GL/RPC7beta. POLR3G/RPC7alpha modulates Pol III transcriptome by specifically enhancing the transcription of snaR-A non-coding RNAs.

1. Canella, D. et al. (2010) Genome research 20, 710-21. PMID: 20413673

2. Girbig, M. et al. (2021) Nature structural & molecular biology 28, 210-219. PMID: 33558764

3. Hou, H. et al. (2021) Nature communications 12, 6135. PMID: 34675218

4. Van Bortle, K. et al. (2022) Nature communications 13, 3007. PMID: 35637192

5. Haurie, V. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 4176-81. PMID: 20154270

6. Wang, Q. et al. (2021) Nature structural & molecular biology 28, 220-227. PMID: 33558766

7. Ablasser, A. et al. (2009) Nature immunology 10, 1065-72. PMID: 19609254

8. Chiu, YH. et al. (2009) Cell 138, 576-91. PMID: 19631370

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-POLR3G polyclonal antibody at 1ug/mL on various samples.
Lane 1: K562 cell lysate

Reférences

Fiche technique

Anti-Human POLR3G Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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