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| カタログ番号 | HT290014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human POLR3G Polyclonal Antibody (HT290014) is a rabbit polyclonal antibody detecting POLR3G in ELISA, IHC, WB. Suitable for Human.
Highlights
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| 種反応性 | Human, Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human POLR3G (Met1-Met110). | ||||||||
| ターゲット | DNA-directed RNA polymerase III subunit RPC7, RNA polymerase III subunit C7, DNA-directed RNA polymerase III subunit G, RNA polymerase III 32 kDa apha subunit, RPC32-alpha, RNA polymerase III 32 kDa subunit, RPC32, POLR3G | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | O15318 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | DNA-directed RNA polymerase III subunit RPC7 (POLR3G) is a ~25 kDa protein. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci. Acts as a long tether that bridges POLR3C/RPC3-POLR3F/RPC6-POLR3G/RPC7 heterotrimer and the mobile stalk of Pol III, coordinating the dynamics of Pol III stalk and clamp modules during the transition from apo to elongation state. Pol III exists as two alternative complexes defined by the mutually exclusive incorporation of subunit POLR3G/RPC7alpha or POLR3GL/RPC7beta. POLR3G/RPC7alpha modulates Pol III transcriptome by specifically enhancing the transcription of snaR-A non-coding RNAs. 1. Canella, D. et al. (2010) Genome research 20, 710-21. PMID: 20413673 2. Girbig, M. et al. (2021) Nature structural & molecular biology 28, 210-219. PMID: 33558764 3. Hou, H. et al. (2021) Nature communications 12, 6135. PMID: 34675218 4. Van Bortle, K. et al. (2022) Nature communications 13, 3007. PMID: 35637192 5. Haurie, V. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 4176-81. PMID: 20154270 6. Wang, Q. et al. (2021) Nature structural & molecular biology 28, 220-227. PMID: 33558766 7. Ablasser, A. et al. (2009) Nature immunology 10, 1065-72. PMID: 19609254 8. Chiu, YH. et al. (2009) Cell 138, 576-91. PMID: 19631370 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-POLR3G polyclonal antibody at 1ug/mL on various samples.
Lane 1: K562 cell lysate
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