Anti-Human PPP1R15A Polyclonal Antibody (HF783014)

Name: Anti-Human PPP1R15A Polyclonal Antibody
Brand: abinScience
SKU: HF783014
Price: 168 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

HF783014

Description

Anti-Human PPP1R15A Polyclonal Antibody (HF783014) is a rabbit polyclonal antibody detecting PPP1R15A in ELISA, IHC, WB. Suitable for Human and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human PPP1R15A (Ala571-Asn621).

Cible

GADD34, Protein phosphatase 1 regulatory subunit 15A, PPP1R15A, Growth arrest and DNA damage-inducible protein GADD34, Myeloid differentiation primary response protein MyD116 homolog

Purification

Purified by antigen affinity column.

Numéro d'accès

O75807

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Protein phosphatase 1 regulatory subunit 15A (PPP1R15A) is a ~73 kDa protein. Recruits the serine/threonine-protein phosphatase PPP1CA to prevents excessive phosphorylation of the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing p53/TP53 phosphorylation on 'Ser-15'. Plays an essential role in autophagy by tuning translation during starvation, thus enabling lysosomal biogenesis and a sustained autophagic flux. Also acts a viral restriction factor by attenuating HIV-1 replication.

1. Choy, MS. et al. (2015) Cell reports 11, 1885-91. PMID: 26095357

2. Santos, CX. et al. (2016) The EMBO journal 35, 319-34. PMID: 26742780

3. Shi, W. et al. (2004) The Journal of cell biology 164, 291-300. PMID: 14718519

4. Yagi, A. et al. (2003) Journal of cellular biochemistry 90, 1242-9. PMID: 14635196

5. Gambardella, G. et al. (2020) Science advances 6. PMID: 32978159

6. Ishaq, M. et al. (2020) Virology 540, 119-131. PMID: 31778897

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Human PPP1R15A Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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