Anti-Human PRKACA Polyclonal Antibody (HB890014)

Name: Anti-Human PRKACA Polyclonal Antibody
Brand: abinScience
SKU: HB890014
Price: 168 USD
Availability: InStock

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Prix(USD) :

Spécification :

50ug
100ug

Nombre :

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HB890014

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human PRKACA (His40-Lys310).

Cible

PRKACA, PKACA, PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha

Purification

Purified by antigen affinity column.

Numéro d'accès

P17612

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

cAMP-dependent protein kinase catalytic subunit alpha (PRKACA) is a ~40 kDa protein. Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, histone H1.4 (H1-4), PSMC5/RPT6, PJA2, RYR2, RORA, SOX9, UHRF1 and VASP. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts.

1. Guan, H. et al. (2005) The Journal of biological chemistry 280, 9957-62. PMID: 15642694

2. D'Souza, T. et al. (2005) The Journal of biological chemistry 280, 26233-40. PMID: 15905176

3. Pasqualucci, L. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 395-400. PMID: 16387847

4. Xu, H. et al. (2008) Breast cancer research and treatment 107, 63-70. PMID: 17333334

5. Zhang, F. et al. (2007) The Journal of biological chemistry 282, 22460-71. PMID: 17565987

6. Xiao, B. et al. (2007) The Journal of biological chemistry 282, 30256-64. PMID: 17693412

7. Shirakawa, K. et al. (2008) Nature structural & molecular biology 15, 1184-91. PMID: 18836454

8. Wang, W. et al. (2010) Molecular and cellular biochemistry 338, 115-22. PMID: 19949837

9. Gambaryan, S. et al. (2010) The Journal of biological chemistry 285, 18352-63. PMID: 20356841

10. Murshid, A. et al. (2010) PloS one 5, e13830. PMID: 21085490

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Human PRKACA Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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