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Anti-Human PRKCSH Polyclonal Antibody (HB154014)

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Anti-Human PRKCSH Polyclonal Antibody
Anti-Human PRKCSH Polyclonal Antibody
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Aperçu
Numéro de catalogueHB154014
Description
Anti-Human PRKCSH Polyclonal Antibody (HB154014) is a rabbit polyclonal antibody detecting PRKCSH in ELISA, IHC, WB. Suitable for Human, Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human PRKCSH (Asp401-Ala511).
Cible Glucosidase II subunit beta, Glucosidase 2 subunit beta, 80K-H protein, G19P1, PKCSH, PRKCSH, Protein kinase C substrate 60.1 kDa protein heavy chain
Purification Purified by antigen affinity column.
Numéro d'accès P14314
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Glucosidase 2 subunit beta (PRKCSH) is a ~59 kDa protein. Regulatory subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins. Required for efficient PKD1/Polycystin-1 biogenesis and trafficking to the plasma membrane of the primary cilia.

1. Pelletier, MF. et al. (2000) Glycobiology 10, 815-27. PMID: 10929008
Note For research use only.
Images
  • Anti-Human PRKCSH Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PRKCSH polyclonal antibody at 1µg/ml on various samples.
    Lane 1: Hela cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: A549 cell lysate
    Lane 5: SKOV3 cell lysate
    Lane 6: Daudi cell lysate
    Lane 7: K562 cell lysate
    Lane 8: U87 cell lysate
    Lane 9: Mouse liver lysate
    Lane 10: Rat liver lysate

Reférences
Fiche technique
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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Anti-Human PRKCSH Polyclonal Antibody

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