Anti-Human PRKCSH Polyclonal Antibody (HB154014)

Name: Anti-Human PRKCSH Polyclonal Antibody
Brand: abinScience
SKU: HB154014
Price: 96 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HB154014

説明

Anti-Human PRKCSH Polyclonal Antibody (HB154014) is a rabbit polyclonal antibody detecting PRKCSH in ELISA, IHC, WB. Suitable for Human, Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human, Mouse

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human PRKCSH (Asp401-Ala511).

ターゲット

Glucosidase II subunit beta, Glucosidase 2 subunit beta, 80K-H protein, G19P1, PKCSH, PRKCSH, Protein kinase C substrate 60.1 kDa protein heavy chain

精製

Purified by antigen affinity column.

アクセッション番号

P14314

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Glucosidase 2 subunit beta (PRKCSH) is a ~59 kDa protein. Regulatory subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins. Required for efficient PKD1/Polycystin-1 biogenesis and trafficking to the plasma membrane of the primary cilia.

1. Pelletier, MF. et al. (2000) Glycobiology 10, 815-27. PMID: 10929008

注意事項

For research use only.

イメージ

Western blot

Western blot analysis was performed using anti-PRKCSH polyclonal antibody at 1µg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: Jurkat cell lysate
Lane 3: HepG2 cell lysate
Lane 4: A549 cell lysate
Lane 5: SKOV3 cell lysate
Lane 6: Daudi cell lysate
Lane 7: K562 cell lysate
Lane 8: U87 cell lysate
Lane 9: Mouse liver lysate
Lane 10: Rat liver lysate

参考文献

データシート

Anti-Human PRKCSH Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

組換えタンパク質

抗体

アッセイキット

Anti-Human PRKCSH Polyclonal Antibody (HB154014)

概要

イメージ

参考文献

データシート

SDS