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Anti-Human PSMB1 Polyclonal Antibody
Anti-Human PSMB1 Polyclonal Antibody
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Обзор
Каталожный номерHB020014
Описание
Anti-Human PSMB1 Polyclonal Antibody (HB020014) is a rabbit polyclonal antibody detecting PSMB1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman, Mouse, Rat
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human PSMB1 (Gly38-Asp241).
Цель PSMB1, Macropain subunit C5, Multicatalytic endopeptidase complex subunit C5, Proteasome gamma chain, Proteasome subunit beta type-1, PSC5, Proteasome component C5
Очистка Purified by antigen affinity column.
Номер доступа P20618
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

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Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Proteasome subunit beta type-1 (PSMB1) is a ~26 kDa protein. Non-catalytic component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation.

Примечание For research use only.
Изображения
  • Anti-Human PSMB1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PSMB1 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: Hela cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: MCF-7 cell lysate
    Lane 4: K562 cell lysate
    Lane 5: THP-1 cell lysate
    Lane 6: U937 cell lysate
    Lane 7: Mouse spleen lysate
    Lane 8: Mouse liver lysate
    Lane 9: Rat spleen lysate
    Lane 10: Rat liver lysate

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Рекомендация

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