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Anti-Human PSMB2 Polyclonal Antibody (HW692014)

Anti-Human PSMB2 Polyclonal Antibody
Anti-Human PSMB2 Polyclonal Antibody
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Vista general
Número de catálogoHW692014
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human PSMB2 (Met1-Ser201).
Objetivo Multicatalytic endopeptidase complex subunit C7-I, Macropain subunit C7-I, Proteasome component C7-I, Proteasome subunit beta type-2, PSMB2
Purificación Purified by antigen affinity column.
Número de acceso P49721
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Proteasome subunit beta type-2 (PSMB2) is a ~22 kDa protein. Non-catalytic component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation.

Nota For research use only.
Imágenes
  • Anti-Human PSMB2 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PSMB2 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: Hela cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: 293T cell lysate
    Lane 4: MCF-7 cell lysate
    Lane 5: K562 cell lysate
    Lane 6: HepG2 cell lysate
    Lane 7: U937 cell lysate

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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