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Anti-Human RABGEF1 Polyclonal Antibody (HP693014)

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Aperçu
Numéro de catalogueHP693014
Description
Anti-Human RABGEF1 Polyclonal Antibody (HP693014) is a rabbit polyclonal antibody detecting RABGEF1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human RABGEF1 (Thr131-Lys368).
Cible RAP1, Rabex-5, RABEX5, Rab5 GDP/GTP exchange factor, RABGEF1, Rabaptin-5-associated exchange factor for Rab5
Purification Purified by antigen affinity column.
Numéro d'accès Q9UJ41
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Rab5 GDP/GTP exchange factor (RABGEF1) is a ~56 kDa protein. Acts as a guanine-nucleotide releasing factor (GEF) for RAB5A by promoting the conversion of inactive RAB-GDP to the active form RAB-GTP. Forms a complex with RABEP1 that acts as Rab4/Rab5 effector regulating endosome fusion. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Also functions as a ubiquitin ligase.

1. Delprato, A. et al. (2004) Cell 118, 607-17. PMID: 15339665
2. Horiuchi, H. et al. (1997) Cell 90, 1149-59. PMID: 9323142
3. Lippé, R. et al. (2001) Molecular biology of the cell 12, 2219-28. PMID: 11452015
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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