Anti-Human RAN Polyclonal Antibody (HX918014)

Name: Anti-Human RAN Polyclonal Antibody
Brand: abinScience
SKU: HX918014
Price: 168 USD
Availability: InStock

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50ug
100ug

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Número de catálogo

HX918014

Descripción

Anti-Human RAN Polyclonal Antibody (HX918014) is a rabbit polyclonal antibody detecting RAN in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human RAN (Met1-Asp171).

Objetivo

GTP-binding nuclear protein Ran, 3.6.5.-, Androgen receptor-associated protein 24, GTPase Ran, Ras-like protein TC4, Ras-related nuclear protein, RAN, ARA24, OK/SW-cl.81

Purificación

Purified by antigen affinity column.

Número de acceso

P62826

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

GTP-binding nuclear protein Ran is a ~24 kDa protein. GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs. Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis. Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN, while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensures the directionality of the transport. Interaction with RANBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins.

1. Hsiao, PW. et al. (1999) The Journal of biological chemistry 274, 20229-34. PMID: 10400640

2. Yamashita, D. et al. (2007) The Journal of biological chemistry 282, 7563-75. PMID: 17209048

3. Rudack, T. et al. (2015) The Journal of biological chemistry 290, 24079-90. PMID: 26272610

4. Aksu, M. et al. (2016) Nature communications 7, 11952. PMID: 27306458

5. Melchior, F. et al. (1993) The Journal of cell biology 123, 1649-59. PMID: 8276887

6. Palacios, I. et al. (1996) The Journal of cell biology 133, 485-94. PMID: 8636225

7. Moroianu, J. et al. (1996) Proceedings of the National Academy of Sciences of the United States of America 93, 7059-62. PMID: 8692944

8. Görlich, D. et al. (1996) The EMBO journal 15, 5584-94. PMID: 8896452

9. Izaurralde, E. et al. (1997) The EMBO journal 16, 6535-47. PMID: 9351834

10. Bischoff, FR. et al. (1997) FEBS letters 419, 249-54. PMID: 9428644

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-Human RAN Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo

Anti-Human RAN Polyclonal Antibody (HX918014)

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Referencias

Datasheet

SDS