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Anti-Human RAP2A Polyclonal Antibody (HY078014)

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Vista general
Número de catálogoHY078014
Descripción
Anti-Human RAP2A Polyclonal Antibody (HY078014) is a rabbit polyclonal antibody detecting RAP2A in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human RAP2A (Met1-Gln183).
Objetivo Ras-related protein Rap-2a, 3.6.5.2, RbBP-30, RAP2A
Purificación Purified by antigen affinity column.
Número de acceso P10114
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Ras-related protein Rap-2a (RAP2A) is a ~20 kDa protein. Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. In its active form interacts with and regulates several effectors including MAP4K4, MINK1 and TNIK. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it is part of several signaling cascades and regulates cytoskeletal rearrangements, cell migration, cell adhesion and cell spreading.

1. Machida, N. et al. (2004) The Journal of biological chemistry 279, 15711-4. PMID: 14966141
2. Taira, K. et al. (2004) The Journal of biological chemistry 279, 49488-96. PMID: 15342639
3. Mittal, V. et al. (2006) The Biochemical journal 394, 309-15. PMID: 16246175
4. Greco, F. et al. (2006) Biochimica et biophysica acta 1763, 330-5. PMID: 16540189
5. Nonaka, H. et al. (2008) Biochemical and biophysical research communications 377, 573-578. PMID: 18930710
6. Kawabe, H. et al. (2010) Neuron 65, 358-72. PMID: 20159449
7. Duncan, ED. et al. (2022) The Journal of cell biology 221. PMID: 35293963
Nota For research use only.
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Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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