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Anti-Human RBM10 Polyclonal Antibody (HX074014)

Anti-Human RBM10 Polyclonal Antibody
Anti-Human RBM10 Polyclonal Antibody
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Aperçu
Numéro de catalogueHX074014
Description
Anti-Human RBM10 Polyclonal Antibody (HX074014) is a rabbit polyclonal antibody detecting RBM10 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human RBM10 (Ser128-Ser207).
Cible GPATC9, S1-1, G patch domain-containing protein 9, RNA-binding protein S1-1, RNA-binding protein 10, GPATCH9, RBM10, RNA-binding motif protein 10, DXS8237E, KIAA0122
Purification Purified by antigen affinity column.
Numéro d'accès P98175
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

RNA-binding protein 10 (RBM10) is a ~103 kDa protein. Binds to ssRNA containing the consensus sequence 5'-AGGUAA-3'. May be involved in post-transcriptional processing, most probably in mRNA splicing. Binds to RNA homopolymers, with a preference for poly(G) and poly(U) and little for poly(A). May bind to specific miRNA hairpins.

1. Nguyen, CD. et al. (2011) Journal of molecular biology 407, 273-83. PMID: 21256132
2. Inoue, A. et al. (2008) Biology of the cell 100, 523-35. PMID: 18315527
3. Treiber, T. et al. (2017) Molecular cell 66, 270-284.e13. PMID: 28431233
Note For research use only.
Images
  • Anti-Human RBM10 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-RBM10 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: K562 cell lysate
    Lane 2: THP-1 cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: 293T cell lysate

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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