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| カタログ番号 | HP626014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human RBM15 Polyclonal Antibody (HP626014) is a rabbit polyclonal antibody detecting RBM15 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
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| 種反応性 | Human, Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human RBM15 (Thr386-Thr529). | ||||||||
| ターゲット | RBM15, RNA-binding motif protein 15, OTT1, One-twenty two protein 1, OTT, RNA-binding protein 15 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q96T37 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | RNA-binding protein 15 (RBM15) is a ~107 kDa protein. RNA-binding protein that acts as a key regulator of N6-methyladenosine (m6A) methylation of RNAs, thereby regulating different processes, such as hematopoietic cell homeostasis, alternative splicing of mRNAs and X chromosome inactivation mediated by Xist RNA. Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing. Plays a key role in m6A methylation, possibly by binding target RNAs and recruiting the WMM complex. Involved in random X inactivation mediated by Xist RNA: acts by binding Xist RNA and recruiting the WMM complex, which mediates m6A methylation, leading to target YTHDC1 reader on Xist RNA and promoting transcription repression activity of Xist. Required for the development of multiple tissues, such as the maintenance of the homeostasis of long-term hematopoietic stem cells and for megakaryocyte (MK) and B-cell differentiation. 1. Patil, DP. et al. (2016) Nature 537, 369-373. PMID: 27602518 2. Zhang, L. et al. (2015) eLife 4. PMID: 26575292 3. Lindtner, S. et al. (2006) The Journal of biological chemistry 281, 36915-28. PMID: 17001072 4. Zolotukhin, AS. et al. (2009) Nucleic acids research 37, 7151-62. PMID: 19786495 5. Mercher, T. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 5776-9. PMID: 11344311 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-RBM15 polyclonal antibody at 1μg/ml on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: K562 cell lysate
Lane 3: Hela cell lysate
Lane 4: 293T cell lysate
Lane 5: Jurkat cell lysate
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