Anti-Human RBX1 Polyclonal Antibody (HX852014)

Name: Anti-Human RBX1 Polyclonal Antibody
Brand: abinScience
SKU: HX852014
Price: 168 USD
Availability: InStock

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100ug

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Каталожный номер

HX852014

Описание

Anti-Human RBX1 Polyclonal Antibody (HX852014) is a rabbit polyclonal antibody detecting RBX1 in ELISA, IHC, WB. Suitable for Human, Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human RBX1 (Ala2-Arg46).

Цель

RING finger protein 75, RNF75, E3 ubiquitin-protein transferase RBX1, N-terminally processed, RBX1, E3 ubiquitin-protein ligase RBX1, Rbx1, ROC1, E3 ubiquitin-protein transferase RBX1, Protein ZYP, RING-box protein 1, Regulator of cullins 1

Очистка

Purified by antigen affinity column.

Номер доступа

P62877

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

E3 ubiquitin-protein ligase RBX1 is a ~12 kDa protein. E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase (CRLs) complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex mediates ubiquitination of Pol II subunit POLR2A at 'Lys-1268', a critical TC-NER checkpoint. Core component of the Cul7-RING(FBXW8) ubiquitin ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.

1. Ohta, T. et al. (1999) Molecular cell 3, 535-41. PMID: 10230407

2. Kamura, T. et al. (1999) Genes & development 13, 2928-33. PMID: 10579999

3. Zheng, N. et al. (2002) Nature 416, 703-9. PMID: 11961546

4. Zhang, DD. et al. (2005) The Journal of biological chemistry 280, 30091-9. PMID: 15983046

5. Wang, H. et al. (2006) Molecular cell 22, 383-94. PMID: 16678110

6. Cen, B. et al. (2009) The Journal of biological chemistry 284, 5265-76. PMID: 19112177

7. Isobe, T. et al. (2009) The Journal of biological chemistry 284, 27766-27779. PMID: 19679664

8. Duda, DM. et al. (2012) Molecular cell 47, 371-82. PMID: 22748924

9. Beck, J. et al. (2013) Nature cell biology 15, 430-9. PMID: 23455478

10. Scott, DC. et al. (2016) Cell 166, 1198-1214.e24. PMID: 27565346

Примечание

For research use only.

Изображения

Ссылки

Даташит

Anti-Human RBX1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

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Anti-Human RBX1 Polyclonal Antibody (HX852014)

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