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Anti-Human RECQL4 Polyclonal Antibody (HF763014)

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Aperçu
Numéro de catalogueHF763014
Description
Anti-Human RECQL4 Polyclonal Antibody (HF763014) is a rabbit polyclonal antibody detecting RECQL4 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human RECQL4 (Gly556-Pro820).
Cible ATP-dependent DNA helicase Q4, RTS, RECQL4, RECQ4, DNA helicase, RecQ-like type 4, RecQ4, RecQ protein-like 4
Purification Purified by antigen affinity column.
Numéro d'accès O94761
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

ATP-dependent DNA helicase Q4 (RECQL4) is a ~133 kDa protein. An ATP-dependent DNA helicase which unwinds dsDNA with a 3'-overhang in a 3'-5' direction. Does not unwind more than 18 bp of dsDNA. May modulate chromosome segregation. The N-terminal domain (residues 1-54) binds DNA Y-shaped DNA better than ss- or dsDNA. The core helicase domain binds ssDNA.

1. Kaiser, S. et al. (2017) Nature communications 8, 15907. PMID: 28653661
2. Ohlenschläger, O. et al. (2012) Nucleic acids research 40, 8309-24. PMID: 22730300
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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