Anti-Human RFWD3 Polyclonal Antibody (HP698014)

Name: Anti-Human RFWD3 Polyclonal Antibody
Brand: abinScience
SKU: HP698014
Price: 168 USD
Availability: InStock

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Precio(USD):

Especificación:

50ug
100ug

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HP698014

Reactividad de especies

Human

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human RFWD3 (Gly430-Glu774).

Objetivo

RING finger and WD repeat domain-containing protein 3, RNF201, E3 ubiquitin-protein ligase RFWD3, RING finger protein 201, RFWD3

Purificación

Purified by antigen affinity column.

Número de acceso

Q6PCD5

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

E3 ubiquitin-protein ligase RFWD3 is a ~85 kDa protein. E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage. Plays a key role in RPA-mediated DNA damage signaling and repair. Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to remove them from DNA damage sites and promote homologous recombination. Also mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome.

1. Gong, Z. et al. (2011) The Journal of biological chemistry 286, 22308-13. PMID: 21504906

2. Liu, S. et al. (2011) The Journal of biological chemistry 286, 22314-22. PMID: 21558276

3. Elia, AE. et al. (2015) Molecular cell 60, 280-93. PMID: 26474068

4. Feeney, L. et al. (2017) Molecular cell 66, 610-621.e4. PMID: 28575657

5. Inano, S. et al. (2017) Molecular cell 66, 622-634.e8. PMID: 28575658

6. Gallina, I. et al. (2021) Molecular cell 81, 442-458.e9. PMID: 33321094

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-Human RFWD3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo