Anti-Human RIOK3 Polyclonal Antibody (HT159014)

Name: Anti-Human RIOK3 Polyclonal Antibody
Brand: abinScience
SKU: HT159014
Price: 96 USD
Availability: InStock

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Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HT159014

Reactividad de especies

Human, Mouse

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human RIOK3 (Ser247-Glu519).

Objetivo

Serine/threonine-protein kinase RIO3, SUDD, sudD homolog, RIO kinase 3, RIOK3

Purificación

Purified by antigen affinity column.

Número de acceso

O14730

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Serine/threonine-protein kinase RIO3 (RIOK3) is a ~59 kDa protein. Serine/threonine-protein kinase involved in a ribosome quality control that takes place when ribosomes have stalled, leading to 18S non-functional rRNA decay and degradation of the 40S ribosomal subunit. Acts downstream of RNF10: specifically recognizes and binds RPS2/us5 and RPS3/us3 monoubiquitinated by RNF10, promoting degradation of the 40S ribosomal subunit in a kinase-dependent manner. The RNF10-RIOK3 ribosome quality control takes place in response to ribosome subunit imbalance or downstream the EIF2AK4/GCN2-mediated integrated stress response (ISR). Also involved in regulation of type I interferon (IFN)-dependent immune response, possibly by acting as an adapter protein essential for the recruitment of TBK1 to IRF3. Phosphorylates IFIH1 on 'Ser-828' interfering with IFIH1 filament assembly on long dsRNA and resulting in attenuated IFIH1-signaling.

1. Coria, AR. et al. (2025) Molecular cell 85, 787-801.e8. PMID: 39947182

2. Huang, Z. et al. (2025) Molecular cell 85, 802-814.e12. PMID: 39947183

3. Ford, PW. et al. (2025) Cell reports 44, 115371. PMID: 40022732

4. Feng, J. et al. (2014) Journal of virology 88, 7987-97. PMID: 24807708

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-RIOK3 polyclonal antibody at 1µg/ml on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: K562 cell lysate
Lane 4: Mouse testis lysate
Lane 5: Rat testis lysate

Referencias

Datasheet

Anti-Human RIOK3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo