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Anti-Human RNF13 Polyclonal Antibody (HT135014)

Anti-Human RNF13 Polyclonal Antibody
Anti-Human RNF13 Polyclonal Antibody
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  • 100ug
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Vista general
Número de catálogoHT135014
Descripción
Anti-Human RNF13 Polyclonal Antibody (HT135014) is a rabbit polyclonal antibody detecting RNF13 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human RNF13 (Glu33-Glu175).
Objetivo E3 ubiquitin-protein ligase RNF13, 2.3.2.27, RING finger protein 13, RNF13, RZF
Purificación Purified by antigen affinity column.
Número de acceso O43567
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

E3 ubiquitin-protein ligase RNF13 is a ~42 kDa protein. E3 ubiquitin-protein ligase that regulates cell proliferation. Involved in apoptosis regulation. Mediates ER stress-induced activation of JNK signaling pathway and apoptosis by promoting ERN1 activation and splicing of XBP1 mRNA. Also involved in protein trafficking and localization.

1. Zhang, Q. et al. (2009) Cell research 19, 348-57. PMID: 18794910
2. Arshad, M. et al. (2013) The Journal of biological chemistry 288, 8726-8736. PMID: 23378536
3. Edvardson, S. et al. (2019) American journal of human genetics 104, 179-185. PMID: 30595371
4. van Dijk, JR. et al. (2014) The Biochemical journal 459, 27-36. PMID: 24387786
Nota For research use only.
Imágenes
  • Anti-Human RNF13 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-RNF13 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse brain lysate
    Lane 2: Rat brain lysate

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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