Anti-Human RNF186 Polyclonal Antibody (HA313014)

Name: Anti-Human RNF186 Polyclonal Antibody
Brand: abinScience
SKU: HA313014
Price: 168 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

HA313014

Description

Anti-Human RNF186 Polyclonal Antibody (HA313014) is a rabbit polyclonal antibody detecting RNF186 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human RNF186 (Gly32-Val150).

Cible

RNF186, RING finger protein 186, E3 ubiquitin-protein ligase RNF186

Purification

Purified by antigen affinity column.

Numéro d'accès

Q9NXI6

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

E3 ubiquitin-protein ligase RNF186 is a ~24 kDa protein. E3 ubiquitin protein ligase that is part of an apoptotic signaling pathway activated by endoplasmic reticulum stress. Stimulates the expression of proteins specific of the unfolded protein response (UPR), ubiquitinates BNIP1 and regulates its localization to the mitochondrion and induces calcium release from the endoplasmic reticulum that ultimately leads to cell apoptosis. Plays a role in the maintenance of intestinal homeostasis and clearance of enteric pathogens. Upon NOD2 stimulation, ubiquitinates the ER stress sensor activating transcription factor 6/ATF6 and promotes the unfolded protein response UPR. Participates in basal level of autophagy maintenance by regulating the ubiquitination of EPHB2 and EPHB3.

1. Wang, P. et al. (2013) Cellular signalling 25, 2320-33. PMID: 23896122

2. Ranjan, K. et al. (2021) The Journal of clinical investigation 131. PMID: 34623328

3. Zhang, H. et al. (2021) Autophagy 17, 3030-3047. PMID: 33280498

4. Lear, TB. et al. (2019) The Journal of biological chemistry 294, 16527-16534. PMID: 31586034

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Human RNF186 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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