Anti-Human ROCK1 Polyclonal Antibody (HC470014)

Name: Anti-Human ROCK1 Polyclonal Antibody
Brand: abinScience
SKU: HC470014
Price: 168 USD
Availability: InStock

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50ug
100ug

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Datasheet
SDS

Vista general

Número de catálogo

HC470014

Descripción

Anti-Human ROCK1 Polyclonal Antibody (HC470014) is a rabbit polyclonal antibody detecting ROCK1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human ROCK1 (Met1-Thr351).

Objetivo

Renal carcinoma antigen NY-REN-35, p160 ROCK-1, p160ROCK, Rho-associated protein kinase 1, Rho-associated, coiled-coil-containing protein kinase I, Rho-associated, coiled-coil-containing protein kinase 1, ROCK-I, ROCK1

Purificación

Purified by antigen affinity column.

Número de acceso

Q13464

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Rho-associated protein kinase 1 (ROCK1) is a ~158 kDa protein. Protein kinase which is a key regulator of the actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, TPPP, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability.

1. Maekawa, M. et al. (1999) Science (New York, N.Y.) 285, 895-8. PMID: 10436159

2. Ohashi, K. et al. (2000) The Journal of biological chemistry 275, 3577-82. PMID: 10652353

3. Sumi, T. et al. (2001) The Journal of biological chemistry 276, 670-6. PMID: 11018042

4. Sebbagh, M. et al. (2001) Nature cell biology 3, 346-52. PMID: 11283607

5. Hagerty, L. et al. (2007) The Journal of biological chemistry 282, 4884-4893. PMID: 17158456

6. Shao, J. et al. (2008) Molecular and cellular biology 28, 5196-208. PMID: 18573880

7. Wang, Y. et al. (2009) Circulation research 104, 531-40. PMID: 19131646

8. Ishizaki, T. et al. (1996) The EMBO journal 15, 1885-93. PMID: 8617235

9. Van Eyk, JE. et al. (1998) The Journal of biological chemistry 273, 23433-9. PMID: 9722579

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-Human ROCK1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

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