Anti-Human ROCK1 Polyclonal Antibody (HC470014)

Name: Anti-Human ROCK1 Polyclonal Antibody
Brand: abinScience
SKU: HC470014
Price: 168 USD
Availability: InStock

データシート製品共有情報コピー

価格(USD):

スペック:

50ug
100ug

数量:

お問い合わせ

概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HC470014

説明

Anti-Human ROCK1 Polyclonal Antibody (HC470014) is a rabbit polyclonal antibody detecting ROCK1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human, Mouse, Rat

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human ROCK1 (Met1-Thr351).

ターゲット

Renal carcinoma antigen NY-REN-35, p160 ROCK-1, p160ROCK, Rho-associated protein kinase 1, Rho-associated, coiled-coil-containing protein kinase I, Rho-associated, coiled-coil-containing protein kinase 1, ROCK-I, ROCK1

精製

Purified by antigen affinity column.

アクセッション番号

Q13464

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Rho-associated protein kinase 1 (ROCK1) is a ~158 kDa protein. Protein kinase which is a key regulator of the actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, TPPP, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability.

1. Maekawa, M. et al. (1999) Science (New York, N.Y.) 285, 895-8. PMID: 10436159

2. Ohashi, K. et al. (2000) The Journal of biological chemistry 275, 3577-82. PMID: 10652353

3. Sumi, T. et al. (2001) The Journal of biological chemistry 276, 670-6. PMID: 11018042

4. Sebbagh, M. et al. (2001) Nature cell biology 3, 346-52. PMID: 11283607

5. Hagerty, L. et al. (2007) The Journal of biological chemistry 282, 4884-4893. PMID: 17158456

6. Shao, J. et al. (2008) Molecular and cellular biology 28, 5196-208. PMID: 18573880

7. Wang, Y. et al. (2009) Circulation research 104, 531-40. PMID: 19131646

8. Ishizaki, T. et al. (1996) The EMBO journal 15, 1885-93. PMID: 8617235

9. Van Eyk, JE. et al. (1998) The Journal of biological chemistry 273, 23433-9. PMID: 9722579

注意事項

For research use only.

イメージ

参考文献

データシート

Anti-Human ROCK1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

組換えタンパク質

抗体

アッセイキット

Anti-Human ROCK1 Polyclonal Antibody (HC470014)

概要

イメージ

参考文献

データシート

SDS

クイックアクセス

お問い合わせ