Anti-Human RPA2 Polyclonal Antibody (HB910014)

Name: Anti-Human RPA2 Polyclonal Antibody
Brand: abinScience
SKU: HB910014
Price: 96 USD
Availability: InStock

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HB910014

Реактивность видов

Human

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human RPA2 (Thr50-Glu270).

Цель

RPA34, Replication protein A 34 kDa subunit, Replication protein A 32 kDa subunit, RF-A protein 2, RP-A p34, Replication factor A protein 2, REPA2, RPA2, RPA32, RP-A p32

Очистка

Purified by antigen affinity column.

Номер доступа

P15927

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Replication protein A 32 kDa subunit (RPA2) is a ~29 kDa protein. As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response.

1. Gupta, R. et al. (2007) Blood 110, 2390-8. PMID: 17596542

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-RPA2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: SH-SY5Y cell lysate
Lane 2: Hela cell lysate
Lane 3: Jurkat cell lysate
Lane 4: MOLT-4 cell lysate
Lane 5: THP-1 cell lysate
Lane 6: Lncap cell lystae
Lane 7: 293T cell lysate
Western blot

Western blot analysis was performed using anti-RPA2 polyclonal antibody at 1μg/ml on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: Hela cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Molt-4 cell lysate
Lane 5: THP-1 cell lysate
Lane 6: Lncap cell lysate
Lane 7: 293T cell lysate

Ссылки

Даташит

Anti-Human RPA2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа