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Anti-Human RPL23A Polyclonal Antibody (HX936014)

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Anti-Human RPL23A Polyclonal Antibody
Anti-Human RPL23A Polyclonal Antibody
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Vista general
Número de catálogoHX936014
Descripción
Anti-Human RPL23A Polyclonal Antibody (HX936014) is a rabbit polyclonal antibody detecting RPL23A in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human RPL23A (Lys70-Ile156).
Objetivo Large ribosomal subunit protein uL23, RPL23A, 60S ribosomal protein L23a
Purificación Purified by antigen affinity column.
Número de acceso P62750
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Large ribosomal subunit protein uL23 (RPL23A) is a ~17 kDa protein. Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Binds a specific region on the 26S rRNA. May promote p53/TP53 degradation possibly through the stimulation of MDM2-mediated TP53 polyubiquitination.

1. Anger, AM. et al. (2013) Nature 497, 80-5. PMID: 23636399
2. Liang, X. et al. (2020) Nature communications 11, 3542. PMID: 32669547
3. Datta, D. et al. (2015) The Journal of biological chemistry 290, 21536-52. PMID: 26203195
Nota For research use only.
Imágenes
  • Anti-Human RPL23A Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-RPL23A polyclonal antibody at 1ug/mL on various samples.
    Lane 1: 293T cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: U87 cell lysate
    Lane 4: Mouse brain lysate
    Lane 5: Mouse lung lysate
    Lane 6: Mouse liver lysate
    Lane 7: Rat brain lysate
    Lane 8: Rat lung lysate
    Lane 9: Rat liver lysate

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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