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Anti-Human RPS3 Polyclonal Antibody (HB915014)

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Vista general
Número de catálogoHB915014
Descripción
Anti-Human RPS3 Polyclonal Antibody (HB915014) is a rabbit polyclonal antibody detecting RPS3 in ELISA, IHC, WB. Suitable for Ambystoma mexicanum, Bovine, Dog, Human, and Ictalurus punctatus.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human RPS3 (Met1-Lys187).
Objetivo Small ribosomal subunit protein uS3, RPS3, 40S ribosomal protein S3
Purificación Purified by antigen affinity column.
Número de acceso P23396
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Small ribosomal subunit protein uS3 (RPS3) is a ~26 kDa protein. Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Has endonuclease activity and plays a role in repair of damaged DNA. Cleaves phosphodiester bonds of DNAs containing altered bases with broad specificity and cleaves supercoiled DNA more efficiently than relaxed DNA. Displays high binding affinity for 7,8-dihydro-8-oxoguanine (8-oxoG), a common DNA lesion caused by reactive oxygen species (ROS).

1. Anger, AM. et al. (2013) Nature 497, 80-5. PMID: 23636399
2. Vladimirov, SN. et al. (1996) European journal of biochemistry 239, 144-9. PMID: 8706699
3. Kim, J. et al. (1995) The Journal of biological chemistry 270, 13620-9. PMID: 7775413
4. Kim, SH. et al. (2005) Biochemical and biophysical research communications 328, 962-7. PMID: 15707971
5. Hegde, V. et al. (2004) DNA repair 3, 121-6. PMID: 14706345
6. Balyeva, KE. et al. (2008) Molekuliarnaia biologiia 42, 314-22. PMID: 18610840
7. Hegde, V. et al. (2004) Biochemistry 43, 14211-7. PMID: 15518571
8. Ko, SI. et al. (2008) Mutation research 648, 54-64. PMID: 18973764
Nota For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Mass
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Volume
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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