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| カタログ番号 | HA855014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human SAE1 Polyclonal Antibody (HA855014) is a rabbit polyclonal antibody detecting SAE1 in ELISA, IHC, WB. Suitable for Human.
Highlights
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| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human SAE1 (Met1-Lys346). | ||||||||
| ターゲット | SAE1, AOS1, Ubiquitin-like 1-activating enzyme E1A, SUA1, SUMO-activating enzyme subunit 1, UBLE1A | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q9UBE0 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | SUMO-activating enzyme subunit 1 (SAE1) is a ~38 kDa protein. The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-SAE1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: NIH3T3 cell lysate
Lane 4: HT-29 cell lysate
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