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Anti-Human SC5D Polyclonal Antibody (HD502014)

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Vista general
Número de catálogoHD502014
Descripción
Anti-Human SC5D Polyclonal Antibody (HD502014) is a rabbit polyclonal antibody detecting SC5D. Suitable for Human.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human SC5D (Ser207-Glu299).
Objetivo C-5 sterol desaturase, Delta(7)-sterol 5-desaturase, Delta(7)-sterol C5(6)-desaturase, EC:1.14.19.20, Lathosterol 5-desaturase, Lathosterol oxidase, SC5D, SC5DL, Sterol-C5-desaturase
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso O75845
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Fondo

Lathosterol oxidase (SC5D) is a ~35 kDa protein. Catalyzes the penultimate step of the biosynthesis of cholesterol, the dehydrogenation of lathosterol into 7-dehydrocholesterol (7-DHC). Cholesterol is the major sterol component in mammalian membranes and a precursor for bile acid and steroid hormone synthesis. In addition to its essential role in cholesterol biosynthesis, it also indirectly regulates ferroptosis through the production of 7-DHC. By diverting the spread of damage caused by peroxyl radicals from the phospholipid components to its sterol nucleus, 7-DHC prevents this form of cell death.

1. Nishi, S. et al. (2000) Biochimica et biophysica acta 1490, 106-8. PMID: 10786622
2. Freitas, FP. et al. (2024) Nature 626, 401-410. PMID: 38297129
3. Li, Y. et al. (2024) Nature 626, 411-418. PMID: 38297130
Nota For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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