Anti-Human SDHB Polyclonal Antibody (HB260014)

Name: Anti-Human SDHB Polyclonal Antibody
Brand: abinScience
SKU: HB260014
Price: 96 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HB260014

説明

Anti-Human SDHB Polyclonal Antibody (HB260014) is a rabbit polyclonal antibody detecting SDHB in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Danio rerio.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human, Mouse, Rat

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human SDHB (Arg38-Val280).

ターゲット

Ip, SDH1, SDHB, Iron-sulfur subunit of complex II, Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, SDH

精製

Purified by antigen affinity column.

アクセッション番号

P21912

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SDHB) is a ~31 kDa protein. Iron-sulfur protein (IP) subunit of the succinate dehydrogenase complex (mitochondrial respiratory chain complex II), responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate. Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate.

1. Ardissone, A. et al. (2015) Molecular genetics and metabolism reports 5, 51-54. PMID: 26925370

2. Grønborg, S. et al. (2017) JIMD reports 33, 69-77. PMID: 27604842

注意事項

For research use only.

イメージ

Western blot

Western blot analysis was performed using anti-SDHB polyclonal antibody at 1μg/ml on various samples.
Lane 1: K562 cell lysate
Lane 2: Mouse brain lysate
Lane 3: Mouse heart lysate
Lane 4: Mouse liver lysate
Lane 5: Rat brain lysate
Lane 6: Rat heart lysate
Lane 7: Rat liver lysate

参考文献

データシート

Anti-Human SDHB Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

組換えタンパク質

抗体

アッセイキット

Anti-Human SDHB Polyclonal Antibody (HB260014)

概要

イメージ

参考文献

データシート

SDS

クイックアクセス

お問い合わせ