Anti-Human SDHB Polyclonal Antibody (HB260014)

Name: Anti-Human SDHB Polyclonal Antibody
Brand: abinScience
SKU: HB260014
Price: 96 USD
Availability: InStock

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Обзор

Каталожный номер

HB260014

Описание

Anti-Human SDHB Polyclonal Antibody (HB260014) is a rabbit polyclonal antibody detecting SDHB in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Danio rerio.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse, Rat

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human SDHB (Arg38-Val280).

Цель

Ip, SDH1, SDHB, Iron-sulfur subunit of complex II, Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, SDH

Очистка

Purified by antigen affinity column.

Номер доступа

P21912

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SDHB) is a ~31 kDa protein. Iron-sulfur protein (IP) subunit of the succinate dehydrogenase complex (mitochondrial respiratory chain complex II), responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate. Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate.

1. Ardissone, A. et al. (2015) Molecular genetics and metabolism reports 5, 51-54. PMID: 26925370

2. Grønborg, S. et al. (2017) JIMD reports 33, 69-77. PMID: 27604842

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-SDHB polyclonal antibody at 1μg/ml on various samples.
Lane 1: K562 cell lysate
Lane 2: Mouse brain lysate
Lane 3: Mouse heart lysate
Lane 4: Mouse liver lysate
Lane 5: Rat brain lysate
Lane 6: Rat heart lysate
Lane 7: Rat liver lysate

Ссылки

Даташит

Anti-Human SDHB Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

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