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Anti-Human SEC13 Polyclonal Antibody (HX854014)

Anti-Human SEC13 Polyclonal Antibody
Anti-Human SEC13 Polyclonal Antibody
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Aperçu
Numéro de catalogueHX854014
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human SEC13 (Met15-Gln322).
Cible D3S1231E, SEC13L1, SEC13, SEC13-like protein 1, SEC13-related protein, Protein SEC13 homolog, SEC13R, GATOR complex protein SEC13, SEC13A
Purification Purified by antigen affinity column.
Numéro d'accès P55735
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Protein SEC13 homolog is a ~35 kDa protein. Functions as a component of the nuclear pore complex (NPC) and the COPII coat. At the endoplasmic reticulum, SEC13 is involved in the biogenesis of COPII-coated vesicles. Required for the exit of adipsin (CFD/ADN), an adipocyte-secreted protein from the endoplasmic reticulum.

1. Tang, BL. et al. (1997) Molecular and cellular biology 17, 256-66. PMID: 8972206
Note For research use only.
Images
  • Anti-Human SEC13 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-SEC13 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: RAW264.7 cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: Hela cell lysate
    Lane 4: U87 cell lysate
    Lane 5: 293T cell lysate
    Lane 6: MCF-7 cell lysate
    Lane 7: Mouse kidney lysate
    Lane 8: Mouse liver lysate

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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