Anti-Human SETMAR IP Recombinant Antibody (SAA0224) (HS762013)

Name: Anti-Human SETMAR IP Recombinant Antibody (SAA0224)
Brand: abinScience
SKU: HS762013
Price: 191 USD
Availability: InStock

Fiche technique Partager le produit Copier les informations

Prix(USD) :

Spécification :

50ug
100ug
1mg

Nombre :

Contactez-nous

Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HS762013

Reactivité des espèces

Human

Applications

ELISA, IF, IP

Espèce hôte

Mouse

Clonalité

Monoclonal

Isotype

IgG2a

ID de clone

SAA0224

Cible

Histone-lysine N-methyltransferase SETMAR, SETMAR, SET domain and mariner transposase fusion protein, Metnase

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

Q53H47

RRID

Anti-Human SETMAR IP Antibody (SAA0224) (abinScience Cat# HS762013, RRID:AB_3729758)

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Contexte

Histone-lysine N-methyltransferase SETMAR is a ~78 kDa protein. Protein derived from the fusion of a methylase with the transposase of an Hsmar1 transposon that plays a role in DNA double-strand break repair, stalled replication fork restart and DNA integration. DNA-binding protein, it is indirectly recruited to sites of DNA damage through protein-protein interactions. Also has kept a sequence-specific DNA-binding activity recognizing the 19-mer core of the 5'-terminal inverted repeats (TIRs) of the Hsmar1 element and displays a DNA nicking and end joining activity. In parallel, has a histone methyltransferase activity and methylates 'Lys-4' and 'Lys-36' of histone H3. Specifically mediates dimethylation of H3 'Lys-36' at sites of DNA double-strand break and may recruit proteins required for efficient DSB repair through non-homologous end-joining.

1. Lee, SH. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 18075-80. PMID: 16332963

2. Cordaux, R. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 8101-6. PMID: 16672366

3. Miskey, C. et al. (2007) Molecular and cellular biology 27, 4589-600. PMID: 17403897

4. Roman, Y. et al. (2007) Biochemistry 46, 11369-76. PMID: 17877369

5. Beck, BD. et al. (2008) The Journal of biological chemistry 283, 9023-30. PMID: 18263876

6. Goodwin, KD. et al. (2010) Biochemistry 49, 5705-13. PMID: 20521842

7. Hromas, R. et al. (2012) Oncogene 31, 4245-54. PMID: 22231448

8. Kim, HS. et al. (2014) The Journal of biological chemistry 289, 10930-10938. PMID: 24573677

9. Fnu, S. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 540-5. PMID: 21187428

10. Williamson, EA. et al. (2008) Nucleic acids research 36, 5822-31. PMID: 18790802

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human SETMAR IP Antibody (SAA0224)

Reférences

Fiche technique

Anti-Human SETMAR IP Recombinant Antibody (SAA0224).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai