Anti-Human SF3B3 Polyclonal Antibody (HS767014)

Name: Anti-Human SF3B3 Polyclonal Antibody
Brand: abinScience
SKU: HS767014
Price: 168 USD
Availability: InStock

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100ug

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Número de catálogo

HS767014

Descripción

Anti-Human SF3B3 Polyclonal Antibody (HS767014) is a rabbit polyclonal antibody detecting SF3B3 in ELISA, IHC, WB. Suitable for Human, Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human SF3B3 (Met1-Ala362).

Objetivo

STAF130, KIAA0017, SF3B3, Splicing factor 3B subunit 3, SF3b130, Pre-mRNA-splicing factor SF3b 130 kDa subunit, SAP130, SAP 130, Spliceosome-associated protein 130

Purificación

Purified by antigen affinity column.

Número de acceso

Q15393

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Splicing factor 3B subunit 3 (SF3B3) is a ~135 kDa protein. Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3B3 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA. Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.

1. Das, BK. et al. (1999) Molecular and cellular biology 19, 6796-802. PMID: 10490618

2. Das, R. et al. (2000) Molecular cell 5, 779-87. PMID: 10882114

3. Will, CL. et al. (2002) The EMBO journal 21, 4978-88. PMID: 12234937

4. Cretu, C. et al. (2016) Molecular cell 64, 307-319. PMID: 27720643

5. Bertram, K. et al. (2017) Cell 170, 701-713.e11. PMID: 28781166

6. Zhang, Z. et al. (2020) Nature 583, 310-313. PMID: 32494006

7. Tholen, J. et al. (2022) Science (New York, N.Y.) 375, 50-57. PMID: 34822310

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-Human SF3B3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo

Anti-Human SF3B3 Polyclonal Antibody (HS767014)

Vista general

Imágenes

Referencias

Datasheet

SDS