Anti-Human SF3B3 Polyclonal Antibody (HS767014)

Name: Anti-Human SF3B3 Polyclonal Antibody
Brand: abinScience
SKU: HS767014
Price: 168 USD
Availability: InStock

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Spécification :

50ug
100ug

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SDS

Aperçu

Numéro de catalogue

HS767014

Description

Anti-Human SF3B3 Polyclonal Antibody (HS767014) is a rabbit polyclonal antibody detecting SF3B3 in ELISA, IHC, WB. Suitable for Human, Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human SF3B3 (Met1-Ala362).

Cible

STAF130, KIAA0017, SF3B3, Splicing factor 3B subunit 3, SF3b130, Pre-mRNA-splicing factor SF3b 130 kDa subunit, SAP130, SAP 130, Spliceosome-associated protein 130

Purification

Purified by antigen affinity column.

Numéro d'accès

Q15393

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Splicing factor 3B subunit 3 (SF3B3) is a ~135 kDa protein. Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3B3 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA. Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.

1. Das, BK. et al. (1999) Molecular and cellular biology 19, 6796-802. PMID: 10490618

2. Das, R. et al. (2000) Molecular cell 5, 779-87. PMID: 10882114

3. Will, CL. et al. (2002) The EMBO journal 21, 4978-88. PMID: 12234937

4. Cretu, C. et al. (2016) Molecular cell 64, 307-319. PMID: 27720643

5. Bertram, K. et al. (2017) Cell 170, 701-713.e11. PMID: 28781166

6. Zhang, Z. et al. (2020) Nature 583, 310-313. PMID: 32494006

7. Tholen, J. et al. (2022) Science (New York, N.Y.) 375, 50-57. PMID: 34822310

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Human SF3B3 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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Kits d'Essai

Anti-Human SF3B3 Polyclonal Antibody (HS767014)

Aperçu

Images

Reférences

Fiche technique

SDS