Anti-Human SF3B3 Polyclonal Antibody (HS767014)

Name: Anti-Human SF3B3 Polyclonal Antibody
Brand: abinScience
SKU: HS767014
Price: 168 USD
Availability: InStock

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Каталожный номер

HS767014

Описание

Anti-Human SF3B3 Polyclonal Antibody (HS767014) is a rabbit polyclonal antibody detecting SF3B3 in ELISA, IHC, WB. Suitable for Human, Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human SF3B3 (Met1-Ala362).

Цель

STAF130, KIAA0017, SF3B3, Splicing factor 3B subunit 3, SF3b130, Pre-mRNA-splicing factor SF3b 130 kDa subunit, SAP130, SAP 130, Spliceosome-associated protein 130

Очистка

Purified by antigen affinity column.

Номер доступа

Q15393

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Splicing factor 3B subunit 3 (SF3B3) is a ~135 kDa protein. Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3B3 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA. Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.

1. Das, BK. et al. (1999) Molecular and cellular biology 19, 6796-802. PMID: 10490618

2. Das, R. et al. (2000) Molecular cell 5, 779-87. PMID: 10882114

3. Will, CL. et al. (2002) The EMBO journal 21, 4978-88. PMID: 12234937

4. Cretu, C. et al. (2016) Molecular cell 64, 307-319. PMID: 27720643

5. Bertram, K. et al. (2017) Cell 170, 701-713.e11. PMID: 28781166

6. Zhang, Z. et al. (2020) Nature 583, 310-313. PMID: 32494006

7. Tholen, J. et al. (2022) Science (New York, N.Y.) 375, 50-57. PMID: 34822310

Примечание

For research use only.

Изображения

Ссылки

Даташит

Anti-Human SF3B3 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-Human SF3B3 Polyclonal Antibody (HS767014)

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