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| カタログ番号 | HB205014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human SFPQ Polyclonal Antibody (HB205014) is a rabbit polyclonal antibody detecting SFPQ in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
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| 種反応性 | Human, Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human SFPQ (Lys271-Gly434). | ||||||||
| ターゲット | Splicing factor, proline- and glutamine-rich, PTB-associated-splicing factor, SFPQ, DNA-binding p52/p100 complex, 100 kDa subunit, Polypyrimidine tract-binding protein-associated-splicing factor, PSF, hPOMp100, 100 kDa DNA-pairing protein | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P23246 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Splicing factor, proline- and glutamine-rich (SFPQ) is a ~76 kDa protein. DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as a heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Involved in regulation of signal-induced alternative splicing. During splicing of PTPRC/CD45, a phosphorylated form is sequestered by THRAP3 from the pre-mRNA in resting T-cells; T-cell activation and subsequent reduced phosphorylation is proposed to lead to release from THRAP3 allowing binding to pre-mRNA splicing regulatotry elements which represses exon inclusion. 1. Lee, M. et al. (2015) Nucleic acids research 43, 3826-40. PMID: 25765647 2. Morchikh, M. et al. (2017) Molecular cell 67, 387-399.e5. PMID: 28712728 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-SFPQ polyclonal antibody at 1μg/ml on various samples.
Lane 1: HepG2 cell lysate
Lane 2: Rat brain lysate
Lane 3: Jurkat cell lysate
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