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Anti-Human SH3RF1 Polyclonal Antibody (HD618014)

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Aperçu
Numéro de catalogueHD618014
Description
Anti-Human SH3RF1 Polyclonal Antibody (HD618014) is a rabbit polyclonal antibody detecting SH3RF1. Suitable for Human, Rat, and Mouse.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human SH3RF1 (Gln135-Lys268).
Cible E3 ubiquitin-protein ligase SH3RF1, EC:2.3.2.27, KIAA1494, POSH, POSH1, Plenty of SH3s, Protein POSH, RING finger protein 142, RING-type E3 ubiquitin transferase SH3RF1, RNF142, SH3 domain-containing RING finger protein 1, SH3 multiple domains protein 2, SH3MD2, SH3RF1
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès Q7Z6J0
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Contexte

E3 ubiquitin-protein ligase SH3RF1 is a ~93 kDa protein. Has E3 ubiquitin-protein ligase activity. In the absence of an external substrate, it can catalyze self-ubiquitination. Stimulates ubiquitination of potassium channel KCNJ1, enhancing its dynamin-dependent and clathrin-independent endocytosis. Acts as a scaffold protein that coordinates with MAPK8IP1/JIP1 in organizing different components of the JNK pathway, including RAC1 or RAC2, MAP3K11/MLK3 or MAP3K7/TAK1, MAP2K7/MKK7, MAPK8/JNK1 and/or MAPK9/JNK2 into a functional multiprotein complex to ensure the effective activation of the JNK signaling pathway. Regulates the differentiation of CD4(+) and CD8(+) T-cells and promotes T-helper 1 (Th1) cell differentiation.

1. Alroy, I. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 1478-83. PMID: 15659549
2. Kärkkäinen, S. et al. (2010) FEBS letters 584, 3867-72. PMID: 20696164
3. Lin, DH. et al. (2009) The Journal of biological chemistry 284, 29614-24. PMID: 19710010
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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