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Anti-Human SIRT3 Polyclonal Antibody (HP409014)

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Vista general
Número de catálogoHP409014
Descripción
Anti-Human SIRT3 Polyclonal Antibody (HP409014) is a rabbit polyclonal antibody detecting SIRT3 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human SIRT3 (Lys122-Lys399).
Objetivo NAD-dependent protein deacetylase sirtuin-3, mitochondrial, SIR2-like protein 3, SIRT3, Regulatory protein SIR2 homolog 3, SIR2L3, hSIRT3
Purificación Purified by antigen affinity column.
Número de acceso Q9NTG7
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

NAD-dependent protein deacetylase sirtuin-3, mitochondrial (SIRT3) is a ~43 kDa protein. NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA, MRPL12 and the ATP synthase subunit ATP5PO. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.

1. Schwer, B. et al. (2002) The Journal of cell biology 158, 647-57. PMID: 12186850
2. Onyango, P. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 13653-8. PMID: 12374852
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4. Schlicker, C. et al. (2008) Journal of molecular biology 382, 790-801. PMID: 18680753
5. Ahn, BH. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 14447-52. PMID: 18794531
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7. Peserico, A. et al. (2013) Cellular and molecular life sciences : CMLS 70, 2015-29. PMID: 23283301
8. Bharathi, SS. et al. (2013) The Journal of biological chemistry 288, 33837-33847. PMID: 24121500
9. Vassilopoulos, A. et al. (2014) Antioxidants & redox signaling 21, 551-64. PMID: 24252090
Nota For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human SIRT3 Polyclonal Antibody

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