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Anti-Human SIRT3 Polyclonal Antibody (HP409014)

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Aperçu
Numéro de catalogueHP409014
Description
Anti-Human SIRT3 Polyclonal Antibody (HP409014) is a rabbit polyclonal antibody detecting SIRT3 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human SIRT3 (Lys122-Lys399).
Cible NAD-dependent protein deacetylase sirtuin-3, mitochondrial, SIR2-like protein 3, SIRT3, Regulatory protein SIR2 homolog 3, SIR2L3, hSIRT3
Purification Purified by antigen affinity column.
Numéro d'accès Q9NTG7
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

NAD-dependent protein deacetylase sirtuin-3, mitochondrial (SIRT3) is a ~43 kDa protein. NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA, MRPL12 and the ATP synthase subunit ATP5PO. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.

1. Schwer, B. et al. (2002) The Journal of cell biology 158, 647-57. PMID: 12186850
2. Onyango, P. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 13653-8. PMID: 12374852
3. Schwer, B. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 10224-10229. PMID: 16788062
4. Schlicker, C. et al. (2008) Journal of molecular biology 382, 790-801. PMID: 18680753
5. Ahn, BH. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 14447-52. PMID: 18794531
6. Jin, L. et al. (2009) The Journal of biological chemistry 284, 24394-405. PMID: 19535340
7. Peserico, A. et al. (2013) Cellular and molecular life sciences : CMLS 70, 2015-29. PMID: 23283301
8. Bharathi, SS. et al. (2013) The Journal of biological chemistry 288, 33837-33847. PMID: 24121500
9. Vassilopoulos, A. et al. (2014) Antioxidants & redox signaling 21, 551-64. PMID: 24252090
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human SIRT3 Polyclonal Antibody

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