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Обзор
Каталожный номерHS734014
Описание
Anti-Human SLC1A5 Polyclonal Antibody (HS734014) is a rabbit polyclonal antibody detecting SLC1A5 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Реактивность видовHuman
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human SLC1A5 (Gln154-Asn230).
Цель Baboon M7 virus receptor, SLC1A5, Solute carrier family 1 member 5, ASCT2, RDR, RD114/simian type D retrovirus receptor, Sodium-dependent neutral amino acid transporter type 2, ATB(0), Neutral amino acid transporter B(0), M7V1, RDRC
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >95% as determined by SDS-PAGE.
Очистка Purified by antigen affinity column.
Номер доступа Q15758
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Neutral amino acid transporter B(0) (SLC1A5) is a ~56 kDa protein. Sodium-coupled antiporter of neutral amino acids. In a tri-substrate transport cycle, exchanges neutral amino acids between the extracellular and intracellular compartments, coupled to the inward cotransport of at least one sodium ion. The preferred substrate is the essential amino acid L-glutamine, a precursor for biosynthesis of proteins, nucleotides and amine sugars as well as an alternative fuel for mitochondrial oxidative phosphorylation. Exchanges L-glutamine with other neutral amino acids such as L-serine, L-threonine and L-asparagine in a bidirectional way. Provides L-glutamine to proliferating stem and activated cells driving the metabolic switch toward cell differentiation.

1. Dun, Y. et al. (2007) Experimental eye research 84, 191-9. PMID: 17094966
2. Pingitore, P. et al. (2013) Biochimica et biophysica acta 1828, 2238-46. PMID: 23756778
3. Scalise, M. et al. (2015) FEBS letters 589, 3617-23. PMID: 26492990
4. Garaeva, AA. et al. (2018) Nature structural & molecular biology 25, 515-521. PMID: 29872227
5. Mazza, T. et al. (2021) FEBS letters 595, 3030-3041. PMID: 34741534
6. Kekuda, R. et al. (1996) The Journal of biological chemistry 271, 18657-61. PMID: 8702519
7. Borowska, AM. et al. (2024) Nature communications 15, 6570. PMID: 39095408
8. Foster, AC. et al. (2016) PloS one 11, e0156551. PMID: 27272177
9. Oburoglu, L. et al. (2014) Cell stem cell 15, 169-84. PMID: 24953180
Примечание For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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