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Anti-Human SLC7A5 Polyclonal Antibody (HC031014)

Anti-Human SLC7A5 Polyclonal Antibody
Anti-Human SLC7A5 Polyclonal Antibody
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Vista general
Número de catálogoHC031014
Descripción
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant human SLC7A5 (Val148-Glu169&Gly215-Asn242&Thr298-Ser324&Lys479-Thr507).
Objetivo Large neutral amino acids transporter small subunit 1; 4F2 light chain; 4F2 LC; 4F2LC; CD98 light chain; Integral membrane protein E16; E16; L-type amino acid transporter 1; hLAT1; Solute carrier family 7 member 5; y+ system cationic amino acid transporter; SLC7A5; CD98LC; LAT1; MPE16
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso Q01650
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Large neutral amino acids transporter small subunit 1 (SLC7A5) is a ~55 kDa protein. The heterodimer with SLC3A2 functions as a sodium-independent, high-affinity transporter that mediates uptake of large neutral amino acids such as phenylalanine, tyrosine, leucine, histidine, methionine, tryptophan, valine, isoleucine and alanine. The heterodimer with SLC3A2 mediates the uptake of L-DOPA. Functions as an amino acid exchanger. May play a role in the transport of L-DOPA across the blood-brain barrier. May act as the major transporter of tyrosine in fibroblasts (Probable).

1. Prasad, PD. et al. (1999) Biochemical and biophysical research communications 255, 283-8. PMID: 10049700
2. Rossier, G. et al. (1999) The Journal of biological chemistry 274, 34948-54. PMID: 10574970
3. Yanagida, O. et al. (2001) Biochimica et biophysica acta 1514, 291-302. PMID: 11557028
4. Friesema, EC. et al. (2001) Endocrinology 142, 4339-48. PMID: 11564694
5. Simmons-Willis, TA. et al. (2002) The Biochemical journal 367, 239-46. PMID: 12117417
6. Kim, DK. et al. (2002) Biochimica et biophysica acta 1565, 112-21. PMID: 12225859
7. Li, S. et al. (2005) The Journal of biological chemistry 280, 20102-10. PMID: 15769744
8. Vumma, R. et al. (2008) Neuroscience letters 434, 56-60. PMID: 18262359
9. Milkereit, R. et al. (2015) Nature communications 6, 7250. PMID: 25998567
10. Yan, R. et al. (2019) Nature 568, 127-130. PMID: 30867591
Nota For research use only
Imágenes
  • Anti-Human SLC7A5 Polyclonal Antibody

    Western blot

    Lane 1: A549 cell lysate
    Lane 2: Hela cell lysate
    Lane 3: MCF-7 cell lysate
    Lane 4: HepG2 cell lysate

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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