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| カタログ番号 | HV523014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human SMURF2 Polyclonal Antibody (HV523014) is a rabbit polyclonal antibody detecting SMURF2 in ELISA, IHC, WB. Suitable for Human.
Highlights
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| 種反応性 | Human, Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human SMURF2 (His246-Glu400). | ||||||||
| ターゲット | SMAD ubiquitination regulatory factor 2, hSMURF2, HECT-type E3 ubiquitin transferase SMURF2, SMURF2, SMAD-specific E3 ubiquitin-protein ligase 2, E3 ubiquitin-protein ligase SMURF2 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q9HAU4 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | E3 ubiquitin-protein ligase SMURF2 is a ~86 kDa protein. E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD7 to trigger SMAD7-mediated transforming growth factor beta/TGF-beta receptor ubiquitin-dependent degradation, thereby down-regulating TGF-beta signaling. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with AIMP1. Also forms a stable complex with TGF-beta receptor-mediated phosphorylated SMAD1, SMAD2 and SMAD3, and targets SMAD1 and SMAD2 for ubiquitination and proteasome-mediated degradation. SMAD2 may recruit substrates, such as SNON, for ubiquitin-dependent degradation. 1. Lin, X. et al. (2000) The Journal of biological chemistry 275, 36818-22. PMID: 11016919 2. Zhang, W. et al. (2023) Molecular cell 83, 4352-4369.e8. PMID: 38016474 3. Kavsak, P. et al. (2000) Molecular cell 6, 1365-75. PMID: 11163210 4. Di Guglielmo, GM. et al. (2003) Nature cell biology 5, 410-21. PMID: 12717440 5. Yan, X. et al. (2011) Molecular and cellular biology 31, 3700-9. PMID: 21791611 6. Lee, YS. et al. (2008) Biochemical and biophysical research communications 371, 395-400. PMID: 18448069 7. Zhang, Y. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 974-9. PMID: 11158580 8. Bonni, S. et al. (2001) Nature cell biology 3, 587-95. PMID: 11389444 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-SMURF2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: MDA-MB-231 cell lysate
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