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Обзор
Каталожный номерHC387014
Описание
Anti-Human SNX17 Polyclonal Antibody (HC387014) is a rabbit polyclonal antibody detecting SNX17 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pongo abelii.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman, Mouse, Rat
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human SNX17 (Met1-Gly401).
Цель KIAA0064, SNX17, Sorting nexin-17
Очистка Purified by antigen affinity column.
Номер доступа Q15036
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Sorting nexin-17 (SNX17) is a ~52 kDa protein. Critical regulator of endosomal recycling of numerous surface proteins, including integrins, signaling receptor and channels. Binds to NPxY sequences in the cytoplasmic tails of target cargos. Associates with retriever and CCC complexes to prevent lysosomal degradation and promote cell surface recycling of numerous cargos such as integrins ITGB1, ITGB5 and their associated alpha subunits. Also required for maintenance of normal cell surface levels of APP and LRP1. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)).

1. Williams, R. et al. (2004) Molecular biology of the cell 15, 3095-105. PMID: 15121882
2. Knauth, P. et al. (2005) Journal of molecular biology 347, 813-25. PMID: 15769472
3. Singla, A. et al. (2024) Nature communications 15, 10193. PMID: 39587083
4. Ghai, R. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 7763-8. PMID: 21512128
5. Steinberg, F. et al. (2012) The Journal of cell biology 197, 219-30. PMID: 22492727
6. McNally, KE. et al. (2017) Nature cell biology 19, 1214-1225. PMID: 28892079
7. Czubayko, M. et al. (2006) Biochemical and biophysical research communications 345, 1264-72. PMID: 16712798
8. Donoso, M. et al. (2009) Molecular biology of the cell 20, 481-97. PMID: 19005208
Примечание For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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